Tehran University of Medical Sciences
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Ghasemali Khorasani

Effects of amniotic membrane extract and deferoxamine on angiogenesis in wound healing: an in vivo model

Authors: Ramyar Farzan, Mahsa Moeinian, Alireza Abdollahi, Zahra Jahangard-Rafsanjani, Abbas Alipour, Marzieh Ebrahimi, Ghasemali Khorasani
Keywords: amniotic membrane extract ● angiogenesis ● deferoxamine ● wound healing
JOURNAL OF WOUND CARE, Vol.27, No.6, 2018,Page:26-32

Objective: Angiogenesis, formation of new vessels from pre-existing
vessels, is an essential part of wound healing. We aimed to compare
amniotic membrane extract with deferoxamine in angiogenesis and to
assess any synergistic effect.
Method: We examined four groups of rats (five per group): control,
deferoxamine, amniotic membrane extract, and deferocxamine and
amniotic membrane extract in combination. A distal-based skin flap was
created. Deferoxamine (100mg/kg), amniotic membrane extract (0.1mg/
ml), and the combination of both were injected subcutaneously every
other day in 10 separate points (0.1 ml at each point) in the skin flap. On
day 11, the animals were euthanised for histopathological evaluation.
Results: Results indicated that the amniotic membrane extract raised
the angiogenic markers, particularly new vessel numbers (p<0.008) and
CD31+ compared with controls (p <0.003), and deferoxamine increased
new vessel numbers and Von Willebrand factor (vWF) significantly
compared with controls (p<0.008). There was an increase in angiogenic
factors in the combined group, however, this was not statistically
significant difference was observed. There was no difference between
amniotic membrane extract and deferoxamine.
Conclusion: Amniotic membrane extract or deferoxamine could be
used interchangeably in angiogenesis within wound healing due to their
high safety and availability.
Declaration of interest: The authors have no conflicts of interest to